|
KMID : 0545120100200050871
|
|
Journal of Microbiology and Biotechnology
2010 Volume.20 No. 5 p.871 ~ p.874
|
|
|
A series of IncQ-based reporter plasmids for use in a range of Gram negative genera
|
|
O’Sullivan Laura E.
Nickerson Cheryl A.
Wilson James W.
|
|
|
Abstract
|
|
|
|
Many studies require expression analysis of the same gene/promoter across a range of bacterial genera. However, there is currently a lack of availability of reporters based on the broad host range IncQ replicon, which is compatible with a popular improved IncP transfer system that is self-transfer defective. We report IncQ lacZ reporter plasmids with features including: (1) compatibility with IncP, IncW, and pBHR/pBBR replicons, (2) a variety of antibiotic markers (Sp-r, Sm-r, Km-r, Cm-r), (3) convenient mobilization via a novel self-transfer-defective IncP conjugation system, and (4) complete DNA sequences. Utility is demonstrated using three different promoters in different Gram negative genera.
|
|
|
KEYWORD
|
|
|
IncQ plasmid, broad host range, IncP, pUZ8002, pQlacZ
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|